- 1). Collect a urine sample from your dog and bring it to your veterinarian. A urine cortisol/creatinine ratio can be performed to check the levels of cortisol in the urine. If this is abnormal, the doctor will likely want to run blood tests.
- 2). Take your dog to the veterinarian, where blood can be drawn for a low-dose dexamethasone suppression test. This test will monitor the cortisol levels in the blood after dexamethasone is given. This test is typically performed when the doctor suspects that the cortisol levels are too high.
- 3). Draw blood for an ACTH stimulation test if the doctor suspects that the cortisol levels are too low. In this test, the dog is given an injection of the ACTH hormone, and blood is taken to determine if the cortisol levels rise or remain the same.
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